Proteins and Peptides
CLDN4 Antibody (LS-B2257)
- 貨號 Cat. No.
- LS-B2257
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2257 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C6153. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Claudin 4 (CLDN4) |
| Synonyms: | CLDN4, claudin 4, cldn4 |
| Family: | Claudin |
| Subfamily: | not assigned-Claudin |
| Host: | CLDN4 antibody was produced in Mouse |
| Antigen Species: | CLDN4 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Synthetic peptide |
| Immunogen: | CLDN4 antibody was raised against synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4 |
| Antibody Specificity: | Recognizes the human ~22kD Claudin 4 protein and does not cross-react with the protein at 55kD. Species cross-reactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry. |
| Antigen Modification: | C-Terminus |
| Predicted Species Reactivity: | Dog, Rat |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2257 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2257 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunofluorescence (IF) (1 - 3 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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