Claudin 12 Antibody (LS-B2181)

貨號 Cat. No.: LS-B2181
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2181
Antibody Validation:	This antibody replaces catalog number LS-C6167. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Claudin 12 (CLDN12)
Synonyms:	CLDN12
Family:	Claudin
Subfamily:	Not assigned
Host:	CLDN12 antibody was produced in Rabbit
Antigen Species:	CLDN12 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	CLDN12 antibody was raised against synthetic peptide derived from the N-terminal region of mouse and human Claudin 12
Antibody Specificity:	Recognizes Claudin 12 protein at ~27kD. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Species sequence homology: human.
Antigen Modification:	N-Terminus
Predicted Species Reactivity:	Mouse
Purification:	Immunoaffinity Chromatography
Presentation:	PBS, pH 7.4, 0.1% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2181 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2181 was determined to be 5 ug/ml. Immunofluorescence: 5 ug/ml, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 3 µg/ml), Immunofluorescence (IF) (5 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.25 mg/ml