Proteins and Peptides
CKS1B Antibody (LS-B2315)
- 貨號 Cat. No.
- LS-B2315
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2315 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C14498. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | CDC28 Protein Kinase 1 (CKS1) (CKS1B) |
| Synonyms: | CKS1B, CDC28 protein kinase 1, cyclin-dependent kinases regulatory subunit 1 |
| Host: | CKS1B antibody was produced in Mouse |
| Antigen Species: | CKS1B antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antigen Type: | Synthetic peptide |
| Immunogen: | CKS1B antibody was raised against synthetic peptide derived from the C-terminal region of the human Cks1 protein. |
| Antibody Specificity: | Recognizes the 11kD human Cks1 protein. No reactivity was observed with a Cks2 recombinant protein. Cross reactivity with an unknown protein at ~35kD has been observed in Western blots of HeLa cell lysates. Reactivity has been confirmed with a Cks1 recombinant protein as well as HeLa cell lysates. IHC reactivity was confirmed with formalin-fixed, paraffin-embedded (FFPE) human tonsil sections. |
| Antigen Modification: | C-Terminus |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2315 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2315 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry (IHC) (50 µg/ml), Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 3 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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