Kits
CIC C3d ELISA
Kits
Description
The importance of the immunocomplex (CIC) and
their relation with several diseases have been object
of investigations for many years.
The enstablishment of immunocomplex is a normal
protecting process of the immune system. The
circulating immunocomplex are removed from the
circulation by means of various cellular, biochemical
and enzymatic processes.
Key of elimination of many CIC is the activation of the
classic way of the complement.
In some diseases, of difficult understanding, the
immunocomplex can begin the damaging of tissue
and organs. In this case the activation of the
complement can lead to the anafilotoxine production,
stimulation of leukocyte and activation of macrophage
and other cells.
In some cases of glomerulonephritis, in which the
immunocomplex fix to the cellular membranes, it has
the destruction of the tissue.
Circulating immuno-complexes (CIC) are present in
many individuals affections from systemic lupus
erythematosus (SLE) and rheumatoid arthritis (RA),
especially in those affections from vasculitis
complications. There are many tests for the
determination of CIC, included the test of precipitation
with PEG, radial immunodiffusion, and cellular tests
like the test of Ray cell.
Does not exist one procedure to determinate all types
of immunocomplex; in commerce some test to
determinate fragments of the complex are available
(Es. C1q and C3d), that have an important diagnostic
meaning. 2. PRINCIPLE
C3d-fixing circulating immune complexes (CIC) are
first blocked by the anti-C3d immobilized on the
microplate. During this phase, the immunocomplex
binds to the antibodies anti C3d coated on the
microplate. The microplate is washed to remove the
unbound serum protein.
In the second phase, the anti human IgG antibodies
conjugated with peroxidase sre added; they bind to
the immunocomplex fixed on the microplate. The
washing solution removes the unbound conjugate. In
the third phase, the TMB Substrate is added, and
reacts with the conjugate fixed on the microplate.
The quantity of CIC IgG complex is proportional to
the colour intensity read at 450 nm wavelengths.
The immunocomplex concentration in the sample is
calculated through a calibration curve. Heat aggregate human gamma globulin per mL
(μgEq/mL) is the unit of measure of the results.
their relation with several diseases have been object
of investigations for many years.
The enstablishment of immunocomplex is a normal
protecting process of the immune system. The
circulating immunocomplex are removed from the
circulation by means of various cellular, biochemical
and enzymatic processes.
Key of elimination of many CIC is the activation of the
classic way of the complement.
In some diseases, of difficult understanding, the
immunocomplex can begin the damaging of tissue
and organs. In this case the activation of the
complement can lead to the anafilotoxine production,
stimulation of leukocyte and activation of macrophage
and other cells.
In some cases of glomerulonephritis, in which the
immunocomplex fix to the cellular membranes, it has
the destruction of the tissue.
Circulating immuno-complexes (CIC) are present in
many individuals affections from systemic lupus
erythematosus (SLE) and rheumatoid arthritis (RA),
especially in those affections from vasculitis
complications. There are many tests for the
determination of CIC, included the test of precipitation
with PEG, radial immunodiffusion, and cellular tests
like the test of Ray cell.
Does not exist one procedure to determinate all types
of immunocomplex; in commerce some test to
determinate fragments of the complex are available
(Es. C1q and C3d), that have an important diagnostic
meaning. 2. PRINCIPLE
C3d-fixing circulating immune complexes (CIC) are
first blocked by the anti-C3d immobilized on the
microplate. During this phase, the immunocomplex
binds to the antibodies anti C3d coated on the
microplate. The microplate is washed to remove the
unbound serum protein.
In the second phase, the anti human IgG antibodies
conjugated with peroxidase sre added; they bind to
the immunocomplex fixed on the microplate. The
washing solution removes the unbound conjugate. In
the third phase, the TMB Substrate is added, and
reacts with the conjugate fixed on the microplate.
The quantity of CIC IgG complex is proportional to
the colour intensity read at 450 nm wavelengths.
The immunocomplex concentration in the sample is
calculated through a calibration curve. Heat aggregate human gamma globulin per mL
(μgEq/mL) is the unit of measure of the results.