Kits
| Article number: | K 6910 |
| Method: | ELISA |
| Tests: | 96 Tests |
| Matrix: | Stool (100mg) |
| Volume: | 100 µl |
| Incubation time: | 3x1h; 30min |
| Standards: | 15.6-1000 ng/ml |
| Chymotrypsin is a serine protease, which is secreted as excretory enzyme from the pancreas after food intake into the duodenum. Here food proteins are hydrolytically cleaved, preferentially next to aromatic residues. A small part of the active form of the enzyme is excreted within the stool. In the case of pancreas insufficiency secondary to a chronic pancreatitis, the secretion of the enzyme is reduced markedly, so that a reduced chymotrypsin activity can be measured in stool specimen.The advantage of this assay is that the concentration of the enzyme is accurately determined and not the enzyme’s activity. Many studies have shown a longer stability of chymotrypsin (12 days at room temperature) compared to pancreatic elastase. Therefore, the chymotrypsin ELISA is a reliable and low-priced alternative to the pancreatic elastase assay. |