Chlortetracycline ELISA Kit

Alternate Name :

Tag Line :

A Competitive ELISA kit for in vitro quantitative determination of Chlortetracycline concentrations in Tissue, Liver, Eggs, Honey, Urine.

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Sample recovery rate: Tissue, Liver, Eggs -90%±20%; Honey -75%±20%; Urine -80±20%, Detection range: Tissue, Liver, Eggs - 0.8 ppb, Honey - 4 ppb, Urine -1 ppb, Sensitivity: 0.1 ppb, Cross-reactivity: Chlortetracycline - 100%, Tetracycline -29%, Oxytetracycline -15%, Doxycycline -2.5%

Description:

Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. It inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. It is effective against Gram-positive and to a lesser degree Gram-negative bacteria than tetracycline. BioVision Chlortetracycline ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Chlortetracycline. During the reaction, Chlortetracycline in the samples or standard competes with Chlortetracycline coated on the plate for binding to the anti-Chlortetracycline antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Chlortetracycline. The concentration of Chlortetracycline in the samples can be calculated by comparing the OD of the samples to the standard curve.