Chicken polyclonal antibody to Microtubule Associated Protein 2 (MAP2)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Microtubule-associated protein 2; MAP-2; MAP2;

 

Antibody_Isotype
(Antibody only) : IgY

 

Application_Details :

Western Blotting (WB, Immunocytochemistry (ICC) and Immunohistochemistry (IHC) Suggested dilutions are: 1:10,00-1:20,000 (WB), IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

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Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=997

 

Description (Long Description) : 

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized with 5% trehalose

 

Immunogen (Antigen) :Bovine MAP2 isolated from brain by the GTP microtubule cycling method.

 

Kit_Components :

 

MSDS (File name) :