Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)
Antibodies
- 貨號 Cat. No.
- C-1373-50
- 規格 Size
- 50 µL
- 品牌 Brand
- Biosensis
- Application 應用
- IC 、 IF, Frozen 、 IH(P), Paraffin embedded 、 WB
- Reactivity
- Feline 、 Human 、 Mouse 、 Other Mammals (Predicted) 、 Rat
- Host
- Chicken
- Clonality
- Polyclonal
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
Astrocyte; Glial fibrillary acidic protein; GFAP
Antibody_Isotype
(Antibody only) : IgY
Application_Details :
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:50,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
ICC: 1:1000-1:20,000+, 4% PFA fixed cells in culture, 3hr-o/n incubations, optimization is a must in most cases because of the antibody high reactivity to GFAP
IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=995
Description (Long Description) :
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Expiry_Date :
12 months after purchase
Format (Form) :
Lyophilized with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein.
Immunogen (Antigen) :Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Kit_Components :
MSDS (File name) :
