cGMP Direct Immunoassay Kit (Colorimetric)

Alternate Name :

Tag Line :

Highly Sensitive Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Mammalian
• Applications- The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.

Detection Method:

Features & Benefits:

• Simple procedure
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
• The kit provides a new acetylation procedure to improve detection sensitivity significantly

Description:

Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.