Proteins and Peptides
CFLAR Antibody (LS-B2024)
- 貨號 Cat. No.
- LS-B2024
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2024 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C716. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | CASP8 And FADD-like Apoptosis Regulator (CFLAR) |
| Synonyms: | CFLAR, c-flip, caspase-eight-related protein, caspase-like apoptosis regulatory protein, casper, cflar, cflip, clarp, fadd-like antiapoptotic molecule 1, flame1, flice inhibitory protein, flip, i-flice, inhibitor of flice, mach-related inducer of toxicity, mrit |
| Family: | Apoptosis |
| Subfamily: | Cysteine C14 |
| Host: | CFLAR antibody was produced in Rabbit |
| Antigen Species: | CFLAR antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | CFLAR antibody was raised against synthetic peptide |
| Antibody Specificity: | A peptide corresponding to the aa. 206-222 GRSKEQRLKEQLGAQQE of human I-FLICE was used to immunize rabbits. Anti-I-FLICE antibody was purified by passing the immune serum through peptide column. |
| Antigen Modification: | aa206-222 |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.05% BSA, 0.05% sodium azide. |
| Recommended Storage: | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2024 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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