Cell Transformation Assay Kit (Colorimetric)

Alternate Name :

Tag Line :

Highly Sensitive and Quantitative (Colorimetric), HTP adaptable Assay to measure Cell Transformation

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method - Colorimetric (OD 450 nm)
• Applications: Measure cell transformation in response to stimuli.
- Screen, study, or characterize compounds that influence cell transformation.
- HTP adaptable

Detection Method: Colorimetric (OD 450 nm)

Features & Benefits:

• Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli
• Simple & High throughput-adaptable
• Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

Description:

The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.