Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

Alternate Name :

Tag Line :

Direct assay to measure cell-mediated cytotoxicity,

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (FL1 channel and FL3 channel)
• Applications- Measurement of cell cytotoxicity in response to drug or toxin treatment
• Quantification of the cytotoxic effect of immune effector cells on target cells
• Assessment of physiological mediators and antibodies that affect cell cytotoxicity

Detection Method: Fluorescence (FL1 channel and FL3 channel)

Features & Benefits:

• Direct method to measure cell-mediated cytotoxicity.
• Does not require cell lysis.
• Provides multi-parametric analysis.

Description:

Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.