Proteins and Peptides
CEACAM5 Antibody (LS-B2183)
- 貨號 Cat. No.
- LS-B2183
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2183 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C12418. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Carcinoembryonic Antigen-related Cell Adhesion Molecule 5 (CEACAM5) |
| Synonyms: | CEACAM5, CD66e |
| Family: | CEA |
| Subfamily: | not assigned-CEA |
| Host: | CEACAM5 antibody was produced in Rabbit |
| Antigen Species: | CEACAM5 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Purified protein |
| Immunogen: | CEACAM5 antibody was raised against human CEA isolated from hepatic metastasis of colon adenocarcinoma (180kD). Cellular Localization: Cytoplasmic and luminal membrane. MW of Antigen: 180kD. |
| Antibody Specificity: | Recognizes human CEA and CEA-like proteins such as non-specific cross-reacting antigen (NCA), NCA2, and biliary glycoprotein (BGP1). Has been absorbed with plasma proteins and blood group antigens A and B. Species cross-reactivity: Human and mouse. Weaklyreacts with rat. Does not react with canine. |
| Predicted Species Reactivity: | Mouse |
| Purification: | Purified |
| Presentation: | PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2183 was determined to be 1:100. Immunoprecipitation: 10 ul/mg protein lysate. Use Protein A, native and denatured. Western Blot: 1:200 for 2hrs at RT. Positive control: LS174T cells or colon carcinoma. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:100), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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