CDK2 Antibody (LS-B2482)

貨號 Cat. No.: LS-B2482
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2482
Antibody Validation:	This antibody replaces catalog number LS-C5970. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Cyclin-Dependent Kinase 2 (CDK2)
Synonyms:	CDK2, cdk2, cell division kinase 2, cyclin-dependent kinase 2, p33 protein kinase, p33(CDK2)
Family:	Protein Kinase
Subfamily:	CDC2/CDK
Host:	CDK2 antibody was produced in Mouse
Antigen Species:	CDK2 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG2,k
Antigen Type:	Recombinant protein
Immunogen:	CDK2 antibody was raised against human recombinant cdk2 protein.
Antibody Specificity:	Recognizes human Cdk2. Species cross-reactivity: mouse and rat.
Predicted Species Reactivity:	Mouse, Rat
Purification:	Protein A Column
Presentation:	10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2482 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2482 was determined to be 5 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 ug/ml for 2hrs at RT. Positive control: HeLa cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	0.1 mg/ml