Proteins and Peptides
CDH11 Antibody (LS-B2308)
- 貨號 Cat. No.
- LS-B2308
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2308 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C4286. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Cadherin 11, Type 2, OB-cadherin (osteoblast) (CDH11) |
| Synonyms: | CDH11, 2610005L07Rik, Cad11, cadherin-11, CDHOB, OB, ob-cadherin, OSF-4, OSTEOBLAST CADHERIN, ventral neural cadherin, vnc |
| Family: | Cadherin |
| Subfamily: | not assigned-Cadherin |
| Host: | CDH11 antibody was produced in Rabbit |
| Antigen Species: | CDH11 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | CDH11 antibody was raised against recombinant protein derived from the intracellular domain of the human Cadherin 11 protein. |
| Antibody Specificity: | Reacts most strongly with the ~110kD Cadherin 11 protein. An additional band at ~130kD (presumably the Cadherin 11precursor) may also be observed. Some cross-reactivity with other Cadherins is observed in approximately the following order: Cadherin11>>R-Cadherin>Cadherin 8>>N-Cadherin=P-Cadherin. No cross-reactivity with Cadherin 6 or E-Cadherin has been observed. Species cross-reactivity: Human and mouse. |
| Antigen Modification: | Intracellular |
| Predicted Species Reactivity: | Mouse |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.01% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2308 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2308 was determined to be 5 ug/ml. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr), Western blot (WB) (1 - 2 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.25 mg/ml |
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