Proteins and Peptides
CD74 Antibody (LS-B2598)
- 貨號 Cat. No.
- LS-B2598
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2598 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C5533. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | CD74 |
| Synonyms: | CD74 Antibody, cd74 Antibody, cd74 antigen Antibody, DHLAG Antibody, HLADG Antibody, Ia-GAMMA Antibody, II Antibody, LN-2 Antibody |
| Host: | CD74 antibody was produced in Mouse |
| Antigen Species: | CD74 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antigen Type: | Cells |
| Immunogen: | CD74 antibody was raised against sU-DHL-4 lymphoma cells. |
| Antibody Specificity: | Recognizes the invariant chain of the HLA-DR antigen (CD74) at 35kD. Species cross-reactivity: Human and baboon. Does not react with rat. |
| Predicted Species Reactivity: | Baboon |
| Non-reactive Species: | Rat |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2598 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2598 was determined to be 5 ug/ml. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells, tonsil, and lymph node. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml), Flow Cytometry (Flo), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |


