Proteins and Peptides
CD74 Antibody (LS-B2594)
- 貨號 Cat. No.
- LS-B2594
- 規格 Size
- 250 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2594 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C5521. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | CD74 |
| Synonyms: | CD74 Antibody, cd74 Antibody, cd74 antigen Antibody, DHLAG Antibody, HLADG Antibody, Ia-GAMMA Antibody, II Antibody, LN-2 Antibody |
| Host: | CD74 antibody was produced in Mouse |
| Antigen Species: | CD74 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1,k |
| Antigen Type: | Cells |
| Immunogen: | CD74 antibody was raised against sU-DHL-4 lymphoma cells. Cellular Localization: Cell membrane. |
| Antibody Specificity: | Recognizes human CD74 at 35kD. Recognizes B-lymphocytes and many B-cell lymphomas. Species cross-reactivity: Baboon. Does not react with rat. |
| Predicted Species Reactivity: | Baboon |
| Non-reactive Species: | Rat |
| Purification: | Protein G Column |
| Presentation: | 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2594 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2594 was determined to be 10 ug/ml. Western Blot: 1-2 ug/ml for 2 hours at RT. Positive control: Raji cells. Tonsil/lymph node. |
| Antibody Uses: | Immunohistochemistry (IHC) (2 - 4 µg/ml), Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml), Flow Cytometry (Flo), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 250 µl |
| Concentration: | 0.1 mg/ml |
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