CD62E Antibody (LS-B2323)

貨號 Cat. No.: LS-B2323
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2323
Antibody Validation:	This antibody replaces catalog number LS-C48574. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Selectin E (endothelial Adhesion Molecule 1) (SELE)
Synonyms:	SELE, cd62e, e-selectin, elam-1, elam1, endothelial leukocyte adhesion molecule 1, lecam2, leukocyte-endothelial cell adhesion molecule 2, sele
Host:	SELE antibody was produced in Rabbit
Antigen Species:	SELE antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	SELE antibody was raised against synthetic peptide surrounding amino acid 584 of human E- Selectin
Antibody Specificity:	The antibody recognizes 67 kD E-Selectin in samples from human, mouse, and rat origins. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysate can be used as positive controls. Blocking Peptide is available separately.
Predicted Species Reactivity:	Mouse, Rat
Purification:	Protein A Column
Presentation:	PBS, 0.02% Thimerosal.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2323 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2323 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 4 µg/ml), Immunoprecipitation (IP) (10 - 20 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml