CD5 Antibody

h1 class=titleCD5 Antibody/h1
div id=node-84 class=node ntype-antibodiesMouse Monoclonal, FITC-conjugated
div class=contentstyle type=text/css
!--
h4 {
margin: 30px 0 0 0;
padding: 0;
border-top: 1px solid #bbb;
background: #f8f8f8;
}
--/style
div class=product-table
table border=0 cellspacing=10 cellpadding=0 width=100%
tbody
tr
td valign=top width=50%
h4Catalog Number/h4
pstrong250150/strong/p
h4Unit/h4
pstrong1 ml/strong/p
h4
pnbsp;/p
Format/h4
pEach vial contains 1 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography./p
pnbsp;/p
h4Data and Images/h4
div class=field field-type-text field-field-data-img-01
div class=field-items
div class=field-item
pspan class=inline lefta href=http://www.abbiotec.com/image/fitc-conjugated-cd5-antibodyimg class=image image-medium title=FITC-conjugated CD5 Antibody: The CD5 Antibody (Cat.No.250150) stains human peripheral blood mononuclear cells from lysed whole blood gated on lymphocytes by using flow cytometry. alt=FITC-conjugated CD5 Antibody: The CD5 Antibody (Cat.No.250150) stains human peripheral blood mononuclear cells from lysed whole blood gated on lymphocytes by using flow cytometry. width=222 height=208 src=http://www.abbiotec.com/files/images/3141b701.gif //aspan style=width: 220px class=captionstrongFITC-conjugated CD5 Antibody: /strongThe CD5 Antibody (Cat.No.250150) stains human peripheral blood mononuclear cells from lysed whole blood gated on lymphocytes by using flow cytometry./span/span/p
/div
/div
/div
/td
td valign=top
h4Description/h4
pstrong*** DISCONTINUED PRODUCT, REPLACED WITH CAT. NO. 250994 ***/strong/p
pThis antibody recognizes the CD5 antigen (T1 antigen) which is expressed on all mature T-cells, most thymocytes, and a subset of mature B-cells. Based on stimulatory effects of monoclonal antibodies, CD5 may be involved in signal transduction. Upon T-cell activation, CD5 is phosphorylated on tyrosine residues. CD5 crosslinking induces extracellular calcium mobilisation, tyrosine phosphorylation of intracellular proteins and DAG production. It may serve as a dual receptor, giving stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells, CD5 leads to inhibitory signals, whereas in peripheral mature T-lymphocytes it acts as a costimulatory signal receptor. The antibody can be used for detecting CD5 in flow cytometry, frozen tissue sections, western blotting and immunoprecipitation. It also reacts with rhesus monkey lymphocytes./p
br style=clear: both /
br style=clear: both /
h4Product Citations/h4
pnbsp;/p
h4Recommended Products/h4
pa href=http://www.abbiotec.com/antibodies/CD5-Antibody-0250149 CD5 Antibody/abr /
a href=http://www.abbiotec.com/antibodies/CD5-Antibody250148 CD5 Antibody/abr /
nbsp;/p
h4Alternate Names/h4
pT-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; LEU1/p
h4Accession No./h4
pa class=accession-link target=_blank href=http://au.expasy.org/uniprot/P06127P06127/abr /
a class=accession-link target=_blank href=http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=proteinamp;id=7656965NP_055022/abr /
nbsp;/p
h4Clonality/h4
pCRIS-1/p
h4Antigen/h4
pnbsp;/p
pBalb/c mice were immunized with stimulated human leukocytes./p
pnbsp;/p
h4Application Notes/h4
pnbsp;/p
pFACS with whole blood: 100 mu;l blood + 10 mu;l antibody/p
pFACS with cells: 500,000 cells in 50 mu;l + 10 mu;l antibody/p
pThis antibody recognizes the CD5 antigen (T1 antigen) on all mature T-cells, most thymocytes and on a subset of mature B-cells. In flow cytometry, the antibody stains 50-90% of human peripheral blood mononuclear cells./p
pIP:Use at an assay dependent concentration/p
pWB:Use at an assay dependent concentration/p
pIHC:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :Mk | H/p
pnbsp;/p
h4Storage/h4
pStore at 4deg;C. Product is guaranteed 6 months from the date of shipment./p
/td
/tr
/tbody
/table
/div
/div
/div