CD45 Antibody

h1 class=titleCD45 Antibody/h1
div id=node-131 class=node ntype-antibodiesMouse Monoclonal
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h4Catalog Number/h4
pstrong250177/strong/p
h4Unit/h4
pstrong0.1 mg/strong/p
h4Format/h4
pEach vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography./p
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h4Description/h4
pThis antibody recognizes the CD45 antigen, also named leukocyte common antigen (LCA), since expressed on all leukocytes. The antibody binds an epitope on CD45 named 2b or P. CD45 is a tyrosine phosphatase involved in signal transduction and possibly a regulator of leukocyte activation. The antibody can be used for detecting CD45 in flow cytometry and in immunohistocytology with frozen and paraffin-embedded tissue sections. The epitope 2b on CD45 is resistant to fixation, therefore cells can be pre-fixed before staining with this antibody./p
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h4Product Citations/h4
pnbsp;/p
pMcMichael, AJ et al. (eds), Leukocyte typing III., Oxford University Press, Oxford (1987)/p
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h4Recommended Products/h4
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h4Alternate Names/h4
pLeukocyte common antigen; L-CA; T200; CD45 antigen; PTPRC; Protein tyrosine phosphatase receptor type C/p
h4Accession No./h4
pa class=accession-link target=_blank href=http://au.expasy.org/uniprot/P08575P08575/abr /
a class=accession-link target=_blank href=http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=proteinamp;id=18641347NP_002829/abr /
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h4Clonality/h4
pEO1/p
h4Antigen/h4
pnbsp;/p
h4Application Notes/h4
pnbsp;/p
pFACS with whole blood: 100 mu;l blood + 10 mu;l antibody/p
pFACS with cells: 500,000 cells in 50 mu;l + 10 mu;l antibody/p
pThis antibody recognizes the 2b epitope (also called P epitope) on CD45 antigen. In flow cytometry, the antibody stains gt;95% of human peripheral blood mononuclear cells./p
pIHC:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :H/p
pnbsp;/p
h4Storage/h4
pStore at -20deg;C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment./p
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