CD32B Protein, Human, Recombinant (His Tag)

CD32B/Fcgr2b Protein, Human, Recombinant (His Tag): Product Information

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized human sCD32b-His (CHO) (Cat:10259-H08C) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.18-0.42 μg/ml.

2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His Tag) (Cat. 10259-H08C) bound to Conbercept was 0.3 nM.

Protein Construction

A DNA sequence encoding (Ala46-Pro217) of human CD32b (P31994-1) was expressed,with a C-terminal polyhistidine tag.

Accession#

P31994-1

Expressed Host

CHO Cells

Species

Human

Predicted N Terminal

Ala 46