CD32A Protein, Human, Recombinant (167 Arg, His Tag)

CD32A Protein, Human, Recombinant (167 Arg, His Tag): Product Information

Purity

> 97 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 12.5-200 ng/ml.

2. Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Human, Recombinant (167 Arg, His Tag) (Cat. 10374-H08H) bound to IgG1 Antibody was 1.1 nM.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) precursor was expressed, with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.

Accession#

AAA35827.1

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

Gln 34

Molecule Mass

The recombinant human CD32a consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.