CD3 (HIT3b) Antibody

h1 class=titleCD3 (HIT3b) Antibody/h1
div id=node-1121 class=node ntype-antibodiesPE-conjugated, Mouse Monoclonal
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h4Catalog Number/h4
pstrong251047/strong/p
h4Unit/h4
pstrong2 ml/strong/p
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Format/h4
pEach vial containsnbsp;2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography./p
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h4Description/h4
pThe CD3 (HIT3a) antibody recognizes the 17-19 kD epsilon;-chain of CD3 within the CD3 antigen/T cell antigen receptor (TCR) complex. The CD3 antigen is expressed in the cell cytoplasm during the early stage of T cell development and is expressed on the cell membrane at the late stage. CD3 antigen is displayed on 60-80% of normal peripheral blood lymphocytes and 60-70% of thymocytes and plays an important role in signal transduction after antigen recognition by TCR. The clone HIT3a has a strong mitogenic effect at ng level on T lymphocyte proliferation in soluble or immobilized conditions and has an immunosuppressive effect at high dose. Conversely, the clone HIT3b has a strong mitogenic effect on T lymphocytes proliferation only under immobilized condition. NK cells also express the CD3 antigen in the cytoplasm, making the CD3 (HIT3a) antibody a helpful tool for analyzing the development relationship and the common precursor of both T cells and NK cells./p
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h4Product Citations/h4
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h4Recommended Products/h4
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h4Alternate Names/h4
pT-cell surface glycoprotein CD3; T-cell surface antigen T3/Leu-4; CD3; T3/p
h4Accession No./h4
pa class=accession-link target=_blank href=http://www.expasy.org/uniprot/P07766P07766/abr /
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h4Clonality/h4
pHIT3b/p
h4Antigen/h4
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h4Application Notes/h4
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pFACS with whole blood: 100 mu;l blood + 20 mu;l antibody; FACS with cells: 10E6 cells + 20 mu;l antibody. E: 1:500-1:1,000/p
pFACS: 1:200-1:1,000/p
pIHC: 1:200-1:500/p
pE:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at 4deg;C. Product is guaranteed 6 months from the date of shipment./p
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