CD235ab (HIR2) Antibody

h1 class=titleCD235ab (HIR2) Antibody/h1
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h4Catalog Number/h4
pstrong250984/strong/p
h4Unit/h4
pstrong0.1 mg/strong/p
h4Format/h4
pEach vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography./p
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h4Description/h4
pCD235ab antibody recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoprotein. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation, CD235ab antibody agglutinated untreated RBCs but failed to agglutinate papain-treated cells, and CD235ab antibody HIR2 significantly binds to GPA, but weakly to GPB. HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are characteristically CD235ab positive and CD45 and CD71 all negative./p
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h4Product Citations/h4
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pShen DC., Tang MH., Zhang JX., et al.,nbsp; 1993. HIR2：A monoclonal antibody against glycophorin A.nbsp; J. of Monoclonal Antibody. 1993；9（2）：59/p
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h4Recommended Products/h4
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h4Alternate Names/h4
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h4Accession No./h4
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h4Clonality/h4
pHIR2/p
h4Antigen/h4
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h4Application Notes/h4
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pPurified antibody is suitable for immunohistochemistry with acetone-fixed and frozen sections./p
pE: 1:500-1:1,000/p
pFACS: 1:200-1:1,000/p
pIHC: 1:200-1:500/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at -20deg;C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment./p
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