CD235ab (HIR2) Antibody

h1 class=titleCD235ab (HIR2) Antibody/h1
div id=node-1866 class=node ntype-antibodiesMouse Monoclonal, PE-conjugated, APC-conjugated
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h4Catalog Number/h4
pstrong251096/strong/p
h4Unit/h4
pstrong2 ml/strong/p
h4Format/h4
pEach vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography./p
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h4Description/h4
pCD235ab antibody recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoprotein. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation, CD235ab antibody agglutinated untreated RBCs but failed to agglutinate papain-treated cells, and CD235ab antibody HIR2 significantly binds to GPA, but weakly to GPB. HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are characteristically CD235ab positive and CD45 and CD71 all negative./p
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h4Product Citations/h4
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pShen DC., Tang MH., Zhang JX., et al.,nbsp; 1993. HIR2：A monoclonal antibody against glycophorin A.nbsp; J. of Monoclonal Antibody. 1993；9（2）：59/p
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h4Recommended Products/h4
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h4Alternate Names/h4
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h4Accession No./h4
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h4Clonality/h4
pHIR2/p
h4Antigen/h4
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h4Application Notes/h4
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pFACS with whole blood: 100 mu;l blood + 20 mu;l antibody; FACS with cells: 10E6 cells + 20 mu;l antibody. E: 1:500-1:1,000/p
pFACS: 1:200-1:1,000/p
pIHC: 1:200-1:500/p
pIF:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at 4deg;C. Product is guaranteed 6 months from the date of shipment./p
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