CD235a (HI264) Antibody

h1 class=titleCD235a (HI264) Antibody/h1
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h4Catalog Number/h4
pstrong250983/strong/p
h4Unit/h4
pstrong0.1 mg/strong/p
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Format/h4
pEach vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography./p
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h4Description/h4
pCD235a antibody recognizes glycophorins A (GPA) which is single-pass membrane sialoglycoprotein. GPA is the carrier of blood group M and N specificities, which provides the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Another function for CD235a that has been proposed is as a membrane inhibitor of reactive lysis. CD235a antibody directed to papain-sensitive epitopes located in the extracelluar specific domain of GPA, agglutinating papain-treated cells. GPA is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235a positive and CD45 and CD71 all negative./p
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h4Product Citations/h4
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pDavid M., A. Pascale, B. Armand, et al., 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582./p
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h4Recommended Products/h4
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h4Alternate Names/h4
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h4Accession No./h4
pbr /
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h4Clonality/h4
pHI264/p
h4Antigen/h4
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h4Application Notes/h4
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pPurified antibody is suitable for immunohistochemistry with acetone-fixed and frozen sections./p
pE: 1:500-1:1,000/p
pFACS: 1:200-1:1,000/p
pIHC: 1:200-1:500/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at -20deg;C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment./p
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