CD235a (HI264) Antibody

h1 class=titleCD235a (HI264) Antibody/h1
div id=node-1865 class=node ntype-antibodiesMouse Monoclonal, PE-conjugated
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h4Catalog Number/h4
pstrong251095/strong/p
h4Unit/h4
pstrong2 ml/strong/p
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Format/h4
pEach vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography./p
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h4Description/h4
pCD235a antibody recognizes glycophorins A (GPA) which is single-pass membrane sialoglycoprotein. GPA is the carrier of blood group M and N specificities, which provides the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Another function for CD235a that has been proposed is as a membrane inhibitor of reactive lysis. CD235a antibody directed to papain-sensitive epitopes located in the extracelluar specific domain of GPA, agglutinating papain-treated cells. GPA is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235a positive and CD45 and CD71 all negative./p
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h4Product Citations/h4
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pDavid M., A. Pascale, B. Armand, et al., 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582./p
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h4Recommended Products/h4
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h4Alternate Names/h4
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h4Accession No./h4
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h4Clonality/h4
pHI264/p
h4Antigen/h4
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h4Application Notes/h4
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pFACS with whole blood: 100 mu;l blood + 20 mu;l antibody; FACS with cells: 10E6 cells + 20 mu;l antibody. E: 1:500-1:1,000/p
pFACS: 1:200-1:1,000/p
pIHC: 1:200-1:500/p
pIF:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :H/p
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h4Storage/h4
pStore at 4deg;C. Product is guaranteed 6 months from the date of shipment./p
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