CD16 alpha Antibody

h1 class=titleCD16 alpha Antibody/h1
div id=node-118 class=node ntype-antibodiesMouse Monoclonal
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h4Catalog Number/h4
pstrong250164/strong/p
h4Unit/h4
pstrong0.1 mg /strong/p
h4Format/h4
pEach vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography./p
pnbsp;/p
h4Data and Images/h4
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h4Description/h4
pThis antibody recognizes a polymorphism variant of CD16 alpha antigen, which is a low affinity receptor for aggregated IgG (Fc gamma receptor III). The receptor exists in two distinct forms: a glycosyl phosphatidyl inositol (GPI) form (Fc gamma IIIB), and an integral membrane protein (Fc gamma IIIA). In humans, the transmembrane form is expressed on NK cells and macrophages whereas the GPI-linked form is expressed on granulocytes. This antibody binds both forms but binds poorly to NK cells from donors not carrying the polymorphism. The antibody can be used for detecting CD16 in flow cytometry, immunoprecipitation, western blot and blocks ligand binding./p
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h4Product Citations/h4
pnbsp;/p
pTamm A. and Schmidt R. 1996. J. Immunology. 157: 1576-81/p
pnbsp;/p
h4Recommended Products/h4
pa href=http://www.abbiotec.com/antibodies/CD16-Antibody250165 CD16 Antibody/abr /
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h4Alternate Names/h4
pLow affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-2; Fc-gamma RIII-alpha; Fc-gamma RIIIa; FcRIIIa; Fc-gamma RIII; FcRIII; FcR-10; CD16a antigen; FCGR3A; CD16A; FCG3; FCGR3; IGFR3/p
h4Accession No./h4
pa class=accession-link target=_blank href=http://au.expasy.org/uniprot/P08637P08637/abr /
a class=accession-link target=_blank href=http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=proteinamp;id=50726979NP_000560/abr /
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h4Clonality/h4
pMEM154/p
h4Antigen/h4
pnbsp;/p
h4Application Notes/h4
pnbsp;/p
pFACS with whole blood: 100 mu;l blood + 10 mu;l antibody/p
pFACS with cells: 500,000 cells in 50 mu;l + 10 mu;l antibody/p
pThis antibody recognizes a polymorphism in CD16a antigen, therefore binding poorly to NK cells from certain donors. The antibody blocks ligand binding./p
pWB:Use at an assay dependent concentration/p
pB:Use at an assay dependent concentration/p
pIP:Use at an assay dependent concentration/p
pstrongReacts with/strongnbsp; :H/p
pnbsp;/p
h4Storage/h4
pStore at -20deg;C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment./p
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