CBP (V2427) Antibody

貨號 Cat. No.

BS1078

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse

 

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

CREBBP (V2427) antibody detects endogenous levels of CREBBP protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of CREBBP (V2427) antibody in extracts from HeLa cell treted with Insulin 0.01U/ml 15'.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of CREBBP (V2427) antibody in paraffin-embedded human breast carcinoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy-terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression,even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

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