CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)
Kits
- 貨號 Cat. No.
- K163-100
- 規格 Size
- K163-100
- Sample Type
- Cell and tissue lysates
Description
Alternate Name :
Tag Line :
Specifically detects Caspase-3
Storage Conditions: -20°C
Shelf life: 6-12 months
Summary :
• Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm)
• Species reactivity- Mammalian
• Kit size- 100 assays
• Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates.
Detection Method:
Features & Benefits:
• Simple one-step procedure; takes only ~3 hours
• Fast and convenient
Description:
Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.