CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit

Alternate Name :

Tag Line :

Measures Activated Caspases in intact cells by FACS

Storage Conditions: -20°C

Shelf life: 6-12 months

Summary :

• Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25 and 100 assays)
• Applications- Detection of activated caspases in living cells.

Detection Method:

Features & Benefits:

• Simple one-step procedure; takes only 20-30 minutes
• Fast and convenient
• D₂R is more cell-permeable than other fluorometric caspase substrates

Description:

Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.