Caspase-8 Colorimetric Assay Kit

Alternate Name :

Tag Line :

Convenient, Highly-Sensitive Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method-Absorbance (400 or 405 nm)
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100, 200, 400 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Detection Method:

Features & Benefits:

• Simple one-step procedure; takes 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.

Description:

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.