Caspase 6 Antibody (LS-B2679)

貨號 Cat. No.: LS-B2679
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2679
Antibody Validation:	This antibody replaces catalog number LS-C21311. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Caspase 6 (CASP6)
Synonyms:	CASP6, CASP-6, casp6, caspase 6, mch-2, MCH2
Family:	Protease
Subfamily:	Cysteine C14
Host:	CASP6 antibody was produced in Rabbit
Antigen Species:	CASP6 antibody was raised against Mouse
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide
Immunogen:	CASP6 antibody was raised against synthetic peptide KLH coupled corresponding to residues surrounding the cleavage site of caspase-6.
Antibody Specificity:	Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at Asp194 (15kD). This antibody does not recognize other caspases. Recognizes human, mouse, rabbit.
Antigen Modification:	Cleavage Site
Predicted Species Reactivity:	Human, Rabbit
Purification:	Protein A Column
Presentation:	10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2679 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2679 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:1000), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	0.25 mg/ml