Caspase-4 Fluorometric Assay Kit

Alternate Name :

Tag Line :

Convenient, Highly-Sensitive Assay

Storage Conditions: -20°C

Shelf life: 6-12 months

Summary :

• Detection method- Fluorescence (Ex/Em 400/505 nm)
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100, 200, 400 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Detection Method:

Features & Benefits:

• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.

Description:

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.