Caspase-3 Substrate DEVD-AFC

Product Name: Caspase-3 Substrate DEVD-AFC

Alternate Name/Synonyms: Ac-DEVD-AFC

Appearance: Liquid

Formulation: In DMSO

CAS Number: N/A

Peptide sequence: Ac-Asp-Glu-Val-Asp-AFC

Molecular Formula: C₃₀H₃₃F₃N₅O₁₃

Molecular Weight: 729

Purity: >98% by HPLC

Solubility/Reconstitution Instructions: N/A

Shelf Life: 12 months

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Caution: Potect from light and moisture