Caspase-3 Proform, mouse recombinant

5 ug. The recombinant Procaspase-3 (1183P-5) is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8 (1088-25, -100). We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100 ?l of Reaction Buffer (1068-20) for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits (K105 and K106).