Proteins and Peptides
Caspase 3 Antibody (LS-B2845)
- 貨號 Cat. No.
- LS-B2845
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2845 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C12490. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Caspase 3 (CASP3) |
| Synonyms: | CASP3, apopain, apoptosis-related cysteine protease, casp-3, casp3, Caspase 3, caspase-3, cpp-32, cpp32, CPP32B, cysteine protease cpp32, Procaspase3, sca-1, srebp cleavage activity 1, Yama, yama protein |
| Family: | Protease |
| Subfamily: | Cysteine C14 |
| Host: | CASP3 antibody was produced in Rabbit |
| Antigen Species: | CASP3 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | CASP3 antibody was raised against recombinant full length human Caspase 3 protein. Cellular Localization: Predominantly cytoplasmic with some nuclear staining. |
| Antibody Specificity: | Recognizes human Caspase 3 (CPP32) at 32kD. Species cross-reactivity: mouse, rat, monkey, rabbit, hamster, bovine, sheep, porcine and canine. |
| Predicted Species Reactivity: | Bovine, Dog, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2845 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2845 was determined to be 5 ug/ml. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunoprecipitation (Denatured): 10 ug/mg protein lysate (Use Protein A). Positive control: Jurkat cells and Tonsil. |
| Antibody Uses: | Immunohistochemistry (IHC) (5 - 10 µg/ml), Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (2.5 - 5 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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