Proteins and Peptides
Caspase 3 Antibody (LS-B1501)
- 貨號 Cat. No.
- LS-B1501
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1501 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C11704. It has been validated for use in the following assays: IHC. |
| Antibody Target: | Caspase 3 (CASP3) |
| Synonyms: | CASP3, apopain, apoptosis-related cysteine protease, casp-3, casp3, caspase-3, cpp-32, cpp32, cysteine protease cpp32, sca-1, srebp cleavage activity 1, yama protein |
| Family: | Protease |
| Subfamily: | Cysteine C14 |
| Host: | CASP3 antibody was produced in Rabbit |
| Antigen Species: | CASP3 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | CASP3 antibody was raised against synthetic peptide |
| Antibody Specificity: | Human caspase-3 amino acids 166-183 (TELDSGIETDSGVDDDMA)1 |
| Antigen Modification: | aa166-183 |
| Predicted Species Reactivity: | Baboon, Hamster, Mouse |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | TBS, pH 7.4, 50% glycerol, 0.5 mg/ml BSA, 0.02% sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1501 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1501 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.2 mg/ml |
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