Caspase-12 Fluorometric Assay Kit

Alternate Name :

Tag Line :

Convenient, Highly-Sensitive Assay

Storage Conditions: -20°C

Shelf life: 6-12 months

Summary :

• Detection method- Fluorescence (Ex/Em 400/505)
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Detection Method:

Features & Benefits:

• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.

Description:

Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.