Proteins and Peptides
CASP9 Antibody (LS-B2021)
- 貨號 Cat. No.
- LS-B2021
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2021 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C647. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Caspase 9 (CASP9) |
| Synonyms: | CASP9, apaf3, apoptotic protease activating factor 3, apoptotic protease MCH-6, casp9, caspase-9, ICE-LAP6, ICE-like apoptotic protease 6, mch6 |
| Family: | Protease |
| Subfamily: | Cysteine C14 |
| Host: | CASP9 antibody was produced in Rabbit |
| Antigen Species: | CASP9 antibody was raised against Rat |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | CASP9 antibody was raised against synthetic peptide |
| Antibody Specificity: | A mixture of synthetic peptides corresponding to amino acids 345-359 and 366-383 of rat Caspase-9-CTD. |
| Antigen Modification: | aa345-359 |
| Predicted Species Reactivity: | Human, Mouse |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.02% sodium azide. |
| Recommended Storage: | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2021 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2021 was determined to be 5 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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