Proteins and Peptides
CASP8 Antibody (LS-B2225)
- 貨號 Cat. No.
- LS-B2225
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2225 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C12528. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Caspase 8 (CASP8) |
| Synonyms: | CASP8, casp8, caspase 8, FADD-HOMOLOGOUS ICE/CED3-LIKE PROTEASE, FADD-LIKE ICE, FADD-like interleukin-1beta-converting enzyme, Fas-associated death domain-like IL-1-converting enzyme, FLICE, ICE-like apoptotic protease 5, MCH5, MORT1-ASSOCIATED CED3 HOMOLOG |
| Family: | Protease |
| Subfamily: | Cysteine C14 |
| Host: | CASP8 antibody was produced in Rabbit |
| Antigen Species: | CASP8 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Recombinant protein |
| Immunogen: | CASP8 antibody was raised against recombinant full length human caspase 8 protein |
| Antibody Specificity: | Detects the processed forms (42/44kD, 25kDA and 14kD) of caspase 8. It is the antibody of choice for Immunoprecipitation. Species cross-reactivity: Human, mouse, rat, bovine, monkey. |
| Predicted Species Reactivity: | Bovine, Monkey, Mouse, Rat |
| Purification: | Protein A Column |
| Presentation: | 10 mM PBS, pH 7.4. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2225 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2225 was determined to be 5 ug/ml. Western Blot: 2.5-5 ug/ml for 2 hours at RT. Immunoprecipitation (Denatured): 10 ug/mg protein lysate using Protein A. Positive control: Jurkat cells. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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