CASP1 Antibody (LS-B112)

貨號 Cat. No.: LS-B112
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B112
Antibody Validation:	This antibody replaces catalog number LS-C504. It has been validated for use in the following assays: IHC.
Antibody Target:	Caspase 1 (CASP1)
Synonyms:	CASP1, casp-1, casp1, caspase-1, ICE-like protease, il-1 beta converting enzyme, il-1bc, il1bc, il1bce, interleukin-1 beta convertase, interleukin-1 beta converting enzyme, interleukin-1 beta converting enzyme (ICE)-like protease
Family:	Protease
Subfamily:	Cysteine C14
Host:	CASP1 antibody was produced in Mouse
Antigen Species:	CASP1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1
Antibody Clone Name:	14F468
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	CASP1 antibody was raised against synthetic peptide
Antibody Specificity:	A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. It will cross-react with mouse and rat Caspase-1, based on NCBI BLAST.
Antigen Modification:	aa371-390
Predicted Species Reactivity:	Mouse
Purification:	Protein G Column
Presentation:	PBS, 0.2% gelatin, 0.05% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B112 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B112 was determined to be 20 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (20 µg/ml), Western blot (WB) (5 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml