Western blot - CARD15 antibody (ab52828)
CARD15 antibody
- 貨號 Cat. No.
- ab52828
- 規格 Size
- 100 µl
- 品牌 Brand
- Abcam
Description
CARD15 antibody (ab52828) datasheet
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Product Name | CARD15 antibody | |
Product type | Primary antibodies | |
Description | Mouse polyclonal to CARD15 | |
Immunogen | Recombinant fragment: SHLARRLLDT VWNKGTWACQ KLIAAAQEAQ ADSQSPKLHG CWDPHSLHPA RDLQSHRPAI VRRLHSHVEN MLDLAWERGF VSQYECDEIR LPIFTPSQRA , corresponding to amino acids 82-181 of Human CARD15 | |
Reacts with | Hu | |
| Tested applications | WB | |
| Application notes | Recommended dilutions | |
Cellular localization | Cytoplasmic | |
Relevance | CARD15 (Nod2) is a member of the Nod1/Apaf-1 family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and a C-terminal regulatory domain. CARD15 induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It also confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Unlike Nod1, this homologue is primary expressed in monocytes. Defects in CARD15 are associated with Blau Syndrome (BS) and Crohn's Disease (CD), and are a cause of susceptibility to ulcerative colitis. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein. | |
Raised in | Mouse | |
Clonality | Polyclonal | |
Isotype | IgG | |
Purity | Whole antiserum | |
| Storage buffer | Preservative: None Material safety datasheet (MSDS) for this product: | |
Form | Liquid | |
Concentration | Concentration not determined | |
Storage instructions | Store at -20°C. Stable for 12 months at -20°C | |
CARD15 antibody images: | ||
 All lanes : CARD15 antibody (ab52828) at 1/1000 dilution | ||
