Carcinoembryonic Antigen (CEA) (Human) ELISA Kit

Alternate Name : CD66E, CD66, CEACAM5, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Tag Line :

A sandwich ELISA kit for in vitro quantitative measurement of CEA levels in human serum, plasma and other biological fluids.

Storage Conditions: 4ºC

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Specificity: No Significant cross-reactivity or interference between Human CEA and analogues was observed., Precision: Coefficient of variation is < 10%., Sensitivity: 0.38 ng/mL, Detection Range: 0.63-40 ng/mL

Description:

BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.