Kits
Carbonylated Proteins, ELISA
- 貨號 Cat. No.
- K 7822
- 規格 Size
- 96 Tests
- 品牌 Brand
- IDS
Description
| Article number: | K 7822 |
| Method: | ELISA |
| Tests: | 96 Tests |
| Matrix: | Plasma, cellular and extracellular liquids |
| Volume: | 4 µl |
| Incubation time: | 3h; 20min; 20min; 15min |
| Standards: | 156.25-2500 pmol/mg |
| This ELISA Kit is intended for the determination of protein carbonyls in biological samples such as EDTA-plasma, bronchoalveolar lavage fluid and cerebrospinal fluid, cell extracts and other soluble protein samples. For research use only. Please order additionally to the ELISA: Test for the determination of protein (BCA method) (Art.-Nr. K7822BCA) and special tubes for centrifugation (Art.-Nr. K7822ZR). Reactive oxygen species (ROS) can oxidize proteins, lipids, and DNA, causing damage of their structure and function as well as cell injury. Proteins are oxidized by free radicals, whereby the constituent amino acids are variously modified or degraded. The modifications result in new functional groups such as carbonyl or hydroxyl groups, which may lead to protein fragmentation, formation of protein-protein cross-linkages, disruption of the tertiary structure and loss of functional activity. In addition, ROS are directly associated with diseases like atherosclerosis, rheumatoid arthritis, Alzheimer’s and Parkinson’s disease as well as ageing and cancerogenesis. Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury. The quantity of protein carbonyls in a protein sample can be determined by derivatizing with dinitrophenylhydrazine (DNPH) and measuring the bound anti-DNPH antibodies. The ELISA method enables carbonyls to be measured quantitatively with microgram quantities of protein. |