Canine Primary Small Intestinal Epithelial Cells

Canine Primary Small Intestinal Epithelial Cells from Cell Biologics are isolated from the small intestinal tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Canine Primary Small Intestinal Epithelial Cells are characterized by immunofluorescent staining with ZO-1 antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.