Calpain 11 (M-60)

Calpain 11 (M-75): sc-50504

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BACKGROUND

Calpains are calcium-activated thiol proteases. They are heterodimers with

one large subunit and one small subunit. The large subunit varies between

family members and can be active without the small subunit. Calpains are

involved in intracellular processing of proteins. Calpain 11 is a typical calpain

with four domains, but in place of the typical domain IV, Calpain 11 has a

similar calmodulin-like domain that associates, but does not interact with,

the smaller regulatory subunit. Calpain 11 is found in the testis, localizing to

the spermatozoa during the later stages of meiosis. It is expressed during

spermatogenesis, which suggests that during meiosis and sperm functional

processes, Calpain 11 may regulate calcium-dependent signal transduction

events.

REFERENCES

1. Dear, T.N., et al. 1999. Diverse mRNA expression patterns of the mouse

calpain genes Capn5, Capn6 and Capn11 during development. Mech. Dev.

89: 201-209.

2. Huang, Y., et al. 2001. The calpain family and human disease. Trends Mol.

Med. 7: 355-362.

3. Gafni, J., et al. 2004. Inhibition of calpain cleavage of Huntingtin reduces

toxicity: accumulation of calpain/caspase fragments in the nucleus. J. Biol.

Chem. 279: 20211-20220.

4. Suzuki, K., et al. 2004. Structure, activation and biology of calpain. Diabetes

53: S12-S18.

5. Ben-Aharon, I., et al. 2006. Calpain 11 is unique to mouse spermatogenic

cells. Mol. Reprod. Dev. 73: 767-773.

6. Hou, S.T., et al. 2006. Calpain-cleaved collapsin response mediator protein-3

induces neuronal death after glutamate toxicity and cerebral ischemia.

J. Neurosci. 26: 2241-2249.

7. Saez, M.E., et al. 2006. The therapeutic potential of the calpain family: new

aspects. Drug Discov. Today 11: 917-923.

8. Das, A., et al. 2006. Mechanism of apoptosis with the involvement of calpain

and caspase cascades in human malignant neuroblastoma SH-SY5Y

cells exposed to flavonoids. Int. J. Cancer 119: 2575-2585.

CHROMOSOMAL LOCATION

Genetic locus: CAPN11 (human) mapping to 6p21.1; Capn11 (mouse) mapping

to 17 B3.

SOURCE

Calpain 11 (M-75) is a rabbit polyclonal antibody raised against amino acids

1-75 mapping at the N-terminus of Calpain 11 of mouse origin.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of

shipment. Non-hazardous. No MSDS required.

PRODUCT

Each vial contains 200 μg IgG in 1.0 ml of PBS with < 0.1% sodium azide

and 0.1% gelatin.

APPLICATIONS

Calpain 11 (M-75) is recommended for detection of Calpain 11 of mouse, rat

and human origin by Western Blotting (starting dilution 1:200, dilution range

1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1

ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range

1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-

1:3000).

Suitable for use as control antibody for Calpain 11 siRNA (h): sc-62058, Calpain

11 siRNA (m): sc-62059, Calpain 11 shRNA Plasmid (h): sc-62058-SH,

Calpain 11 shRNA Plasmid (m): sc-62059-SH, Calpain 11 shRNA (h) Lentiviral

Particles: sc-62058-V and Calpain 11 shRNA (m) Lentiviral Particles:

sc-62059-V.

Molecular Weight of Calpain 11: 81 kDa.

Positive Controls: mouse testis extract: sc-2405, rat testis extract: sc-2400

or Calpain 11 (h): 293T lysate: sc-115046.



DATA

RESEARCH USE

For research use only, not for use in diagnostic procedures.