Calciseptine
- 貨號 Cat. No.
- C-500
- 規格 Size
- 70 μg 5x70 μg
Description
Calciseptine
Specific L-type Cav channel blocker
| Product#: | C-500 |
| Sizes: |
| 70 mg |
| 5 x 70 mg |
| M.W.: | 7036 |
| Sequence: | RICYI HKASL PRATK TCVEN TCYKM FIRTQ REYIS ERGCG CPTAM WPYQT ECCKG DRCNK |
| Purity: | >98% |
| Bioassay: |
| Calciseptine was tested for its ability to inhibit L-type Ca2+ channels (Cav1.2, b3) heterologously expressed in Xenopus oocytes. 1 mM Calciseptine blocked 50% of these L-type currents. |
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| References: |
1. | Schweitz, H. et al. (1990) Toxicon 28, 847. |
2. | De Weille, J.R. et al. (1991) PNAS 88, 2437. |
3. | Garcia, M.C. et al. (2001) J. Membr. Biol. 184, 121. |
4. | Teramoto, N. et al. (1996) Pflugers Arch. 432, 462 |
Calciseptine is a natural peptide isolated from the Dendroaspis p. polylepsis black mamba venom, by a modification to the procedure of Schweitz et al. 1 and purified to homogenity. Calciseptine was shown to be a specific L-type Cav channel blocker.2 In contrast, it increases L-type currents in skeletal muscle.3 Calciseptine blocks spontaneous or K+-induced contraction of cardiac and smooth muscle cells.2 Using patch clamp, it was also shown to block specifically L-type Cav channel current in neuronal cells in culture.2 For these variety of effects, the IC50's were in the range of 15-500 nM. Using the outside-out configuration of patch clamp, it was shown to reduce single L-type channels open probability and availability as recorded from Guinea pig portal vein.4