Calcicludine

Calcicludine

Neuronal L-type Ca_v Channel Blocker

Product#:

C-650

Sizes:

70 mg

5 x 70 mg

Calcicludine is a natural peptide isolated from the Dendroaspis angusticeps green mamba venom, by a modification to the procedure of Schweitz et al¹ and purified to homogenity. Calcicludine was shown to be a specific neuronal L-type Ca_v channel blocker and was shown to inhibit other neuronal HVA Ca_v channels (N and P type) as well.¹ 1 mM Calcicludine had no effect on skeletal muscle Ca_v channels (Ca_v1.1) as well as on T-type (Ca_v3) channels in heart and neurons.¹ Calcicludine was tested to confirm its ability to block spontaneous or K⁺-induced contraction of cardiac cells.¹ It blocks Ca_v1.2 cloned channels with IC₅₀ of 88 nM and had very little effect on Ca_v2 family channels.² In addition the block was irreversible upon wash and never complete but reached a maximum of 58%.

M.W.:

6979

Sequence:

WQPPW YCKEP VRIGS CKKQF SSFYF KWTAK KCLPF LFSGC GGNAN RFQTI GECRK KCLGK

Purity:

>98%

Bioassay:

Calcicludine was tested for its ability to inhibit L-type Ca2+channels (Cav1.2, b3) heterologously expressed in Xenopus oocytes. 0.5 mM Calcicludine blocked 50% of these L-type currents.

Left: The effect of 0.5 mM Calcicludine on L-type currents in Xenopus oocytes across the voltage range. Right: Traces of Ca2+ current responses to depolarization to +20 mV before and during application of the toxin.

References:

1.	Schweitz, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 878.
2.	Stotz, S.C. et al. (2000) J. Membr. Biol. 174, 157.