C5AR1 / C5AR Antibody (LS-B2653)

貨號 Cat. No.: LS-B2653
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2653
Antibody Validation:	This antibody replaces catalog number LS-C5672. It has been validated for use in the following assays: IHC-P.
Antibody Target:	C5AR1 / C5AR
Synonyms:	C5AR1 Antibody, C5A Antibody, c5a anaphylatoxin chemotactic receptor Antibody, c5a anaphylatoxin receptor Antibody, c5a receptor Antibody, C5a-R Antibody, c5ar Antibody, c5r1 Antibody, cd88 Antibody, complement component 5 receptor 1 Antibody
Family:	GPCR
Subfamily:	Anaphylatoxin
Host:	C5AR1 antibody was produced in Mouse
Antigen Species:	C5AR1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG2a
Antigen Type:	Synthetic peptide
Immunogen:	C5AR1 antibody was raised against c5aR - peptide: Met1 - Asn31.
Antibody Specificity:	Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone 3H1738 was raised against a synthetic peptide Met1 -Asn31 and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone 3H1738 inhibits C5a binding to its receptor. Species cross-reactivity: Rabbit.
Predicted Species Reactivity:	Rabbit
Purification:	Ion Exchange Chromatography
Presentation:	PBS
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2653 was determined to be 10 ug/ml. Flow Cytometry: 1:50. Use 20 ul to label 5x10^5 cells in 100 ul.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Flow Cytometry (Flo) (1:50), Functional Assay (Func), Neutralization (Neut) (Optimal dilution to be determined by the researcher)
Size:	50 µg